Pengaruh Ekstrak Buah Bakau (Rhizophora sp) terhadap Ekspresi Monocyte Chemoattractant Protein-1 pada Aorta Tikus Aterosklerosis

Muhammad Yulis Hamidy, Huriatul Masdar, Ayu Trisya Arti

Abstract

Atherosclerosis is an atheromatous plaque that protrudes into and clogs blood vessels. This study aimed to analyze the effect of Rhizophora sp extract on the expression of Monocyte Chemoattractant Protein-1 (MCP-1) in the early stages of atherosclerosis in rats fed an atherogenic diet. Eighteen abdominal aortic paraffin blocks of white rats were used, consisting of 3 groups: normal diet (K1), atherogenic diet (K2), and atherogenic diet and Rhizophora sp extract (K3). The effect of Rhizophora sp extract was assessed by immunohistochemical examination. MCP-1 expression was calculated in 5 fields of view, then analyzed using Photoshop Image Analysis. The results of this study indicated that administration of Rhizophora sp extract reduced MCP-1 expression in K3 compared to K2 (0.155±0.075 vs 0.246±0.097) although this result was not statistically significant. In conclusion, Rhizophora sp extract has the ability to inhibit MCP-1 expression in the aorta of rats fed an atherogenic diet.

Keywords

Atherosclerosis, Monocyte Chemoattractant Protein-1 (MCP-1), Rhizophora sp

References

Meidayanti D. Manfaat likopen dalam tomat sebagai pencegahan terhadap timbulnya aterosklerosis. J Med Hutama. 2021;02(03):906–10.

World Health Organisation. Cardiovascular diseases [Internet]. [cited 2022 Jul 1]. Available from: https://www.who.int/health-topics/cardiovascular-diseases#tab=tab_1

Prameswari NP. Pemanfaatan senyawa antiaterogenik jamur tiram putih (Pleurotus spp) dalam pencegahan aterosklerosis. J ilmu mahasiswa ked. Indonesia. Mei-Okober 2019; 7(2) : 61.

Hanna Nurjanah, Budi Setiawan KR. Indonesian Journal of Human Nutrition. Indones J Hum Nutr [Internet]. 2020;7(2):139–52.

Callista O, Handayani D, Tritisari KP. Pengaruh pemberian tepung daun katuk terhadap penebalan dinding aorta tikus wistar. Indonesian Journal of Human Nutrition, Desember 2015, 2(2) : 85 – 90.

Mile L, Nursyam H, Setijawati D, Sulistiyati TD. Studi fitokimia buah mangrove (rhizophora mucronata) di desa langge kabupaten gorontalo utara. Jambura Fish Processing Journal. 2021; 3(1): 1.

Podungge F, Purwaningsih S, Nurhayati T. 2015. The Characteristic of black bakau fruit as extract of antioxidant source. Jurnal Pengolahan Hasil Perikanan Indonesia. 18 (2): 140–149.

Purwaningsih S, Salamah E, Yudha A, Sukarno P, Deskawati E. 2013. Aktivitas antioksidan dari buah mangrove (Rhizophora mucronata Lamk.) pada suhu yang berbeda. Jurnal Pengolahan Hasil Perikanan Indonesia. 16: (3)

Hamidy MY, Masdar H, Darmawi D. Effect of mangrove (rhizophora sp) fruit ekstract on foem cell formation at the initiation stage of atherosclerosis. Biomed Pharmacol J 2020; 13(1).

Soehnlein O. Multiple roles for neutrophils in atherosclerosis. Circ Res 2012;110:875-88.

Aprotosoaie AC, Costache AD, Costache II. Therapeutic strategies and chemoprevention of atherosclerosis: what do we know and where do we go? Pharm 2022, Vol 14, Page 722 [Internet]. 2022 Mar 28 [cited 2022 Jul 2];14(4):722.

Hamidy MY, Masdar H, Darmawi D. Effect of mangrove (rhizophora sp) fruit ekstract on foem cell formation at the initiation stage of atherosclerosis. Biomed Pharmacol J 2020; 13(1).

Harsa IMS. Efek pemberian diet tinggi lemak terhadap profil lemak darah tikus putih (Rattus norvegicus). Univ Wijaya Kusuma. 2014;31(1):21–8.

Ismawati, Oenzil F, Yanwirasti, Yerizel E. Changes in expression of proteasome in rats at different stages of atherosclerosis. Anat Cell Biol. 2016;49(2):99–106.

Alfarisi HAH, Mohamed ZBH, Ibrahim M Bin. Basic pathogenic mechanisms of atherosclerosis. Egypt J Basic Appl Sci [Internet]. 2020;7(1):116–25.

Zhang ZK, Li J, Yan DX, Leung WN, Zhang BT. Icaritin inhibits collagen degradation-related factors and facilitates collagen accumulation in atherosclerotic lesions: A potential action for plaque stabilization. Int J Mol Sci. 2016;17(2):1–16.

Bergheanu, SC. Bodde, MC. Jukema, JW. Patofisiologi dan pengobatan aterosklerosis. Neth Heart J. 2017 Apr; 25(4): 231–242.

Jebari-Benslaiman S, Galicia-García U, Larrea-Sebal A, Olaetxea JR, Alloza I, Vandenbroeck K, et al. Pathophysiology of Atherosclerosis. Int J Mol Sci. 2022;23(6):1–38.

Marchio P, Guerra-Ojeda S, Vila JM, Aldasoro M, Victor VM, Mauricio MD. Targeting early atherosclerosis: A focus on oxidative stress and inflammation. Oxid Med Cell Longev. 2019;2019(Ldl):1–32.

Hamidy MY, Masdar H, Winarto W. Mangrove (Rhizophora sp.) fruit extract inhibits tumor growth factor (TGF)-β1 expression in high cholesterol diet-fed rats. J Farm Galen (Galenika J Pharmacy). 2021;7(3):213–20.

Hamidy MY, Oenzil F, Yanwirasti, Aldi Y. Effect of andrographolide on monocyte chemoattractant protein-1 expression at the initiation stage of atherosclerosis in atherogenic diet-fed rats. Biomed Pharmacol J. 2019;12(3):1167–73.

Banjarnahor, S. D. S., Artanti, N. Antioxidant properties of flavonoids. Medical Journal of Indonesia. 2014; 23(4), 239–244.

Treml J, Šmejkal K. Flavonoids as Potent Scavengers of Hydroxyl Radicals. Compr Rev Food Sci Food Saf [Internet]. 2016 Jul 1 [cited 2022 Nov 8];15(4):720–38.

Hamidy MY, Oenzil F, Yanwirasti, Aldi Y. Effect of andrographolide on monocyte chemoattractant protein-1 expression at the initiation stage of atherosclerosis in atherogenic diet-fed rats. Biomed Pharmacol J. 2019;12(3):1167–73.

Maulita SD, Santosa PE, Suharyati S, Hartono M, Tantalo S. The profile of avian influenza and newcastle disease antibody titers on rooster by giving sambiloto extract treatment. 2022 [cited 2022 Nov 9];6(4):360–7

DOI: https://doi.org/10.26891/JIK.v17i1.2023.41-45

Refbacks

  • There are currently no refbacks.