Validitas Metode Konvensional Modifikasi Terhadap Metode Konvensional Dan ChromidTM Esbl Untuk Deteksi Bakteri-bakteri Penghasil Extended-spectrum Beta-lactamases

Abstract

Extended spectrum beta lactamases (ESBL) enzymes producing microorganisms are a major problem in increasing
of betalactamases antibiotics resistance include cephalosporins. These enzymes are produced by gram negatives
bacilli especially a variety of Enterobacteriaceae, however the most common ESBL producing microorganisms are
Klebsiella spp and Escherichia coli. Detection and identification of gram negatives bacilli producing ESBL are
challenging for clinical microbiology laboratory to determine the best method to get the optimal results. The objective
of this study was to determine the sensitivity and specificity of conventional method modification using MacConkey
added with cefpodoxim disc 10 μg, 2 mg/l to conventional method and ChromIDTM ESBL. The method of this study
was an observasional analytical study using cross sectional design on 200 isolates, which were Escherichia coli,
Klebsiella spp, Salmonella typhi, Proteus mirabilis, Pseudomonas aeruginosa, Serratia spp, and Enterobacter spp.
The sensitivity of conventional method modification was 93,8%, conventional method was 68,8% and ChromIDTM
ESBL method was 100%. The spesificity of conventional method modification was 98,7%, conventional method was
11,2% and ChromIDTM ESBL method was 100%. The sensitivity of conventional method modification was higher
than conventional method and as good as ChromIDTM ESBL for detecting ESBL producing bacterias.