Pengaruh Asam Alfa Lipoat Terhadap Kadar Trigliserida pada Tikus Diabetes Melitus Tipe 2

Angel Chen, Ismawati Ismawati, Enikarmila Asni

Abstract

Accumulation of triglycerides associated with  insulin resistance in type 2 diabetes mellitus (T2DM). Alpha lipoic acid (ALA) has hypotriglyceridemic effects by lowering triglyceride levels in blood and liver. This study aims to analyze effect of ALA on triglyceride levels in  type 2 diabetic rats. Fifteen Wistar male rats (±8-12 weeks, 200-250 gram) divided into 3 groups (control, T2DM  and  T2DM–ALA given for 3 weeks). Diabetes was induced by giving streptozotocin (50 mg/kg.bw) and nicotinamide (110 mg/kg.bw) after 15 minutes intraperitoneally. Diagnosis of T2DM was determined if the rats have perifer blood glucose >250 mg/dL after 3 days. The results showed that triglyceride levels in  ALA group (p=0,003) lower than T2DM group, T2DM group (p=0,031)  had higher triglyceride level than control and no difference of triglyceride level between  T2DM-ALA given group with control (p=0,696). This study concluded that ALA can affect triglyceride levels in T2DM thus prevents occurrence of hypertriglyceridemia.

Keywords

alpha lipoic acid, triglyceride, type 2 diabetes mellitus, streptozotocin, nicotinamid

Full Text:

PDF

References

Diez JMB, Pennafiel J, Subirana I, Ramos R, Elosua R, Ibanez AM, et al. Risk of cause-specific death in individuals with diabetes: A competing risks analysis. Diabetes Care. 2016;39(11):1987–95.

Badan Penelitian dan Pengembangan Kesehatan. Riset Kesehatan Dasar (RISKESDAS) 2013. Lap Nas 2013. 2013;1–384.

Gaede P, Oellgaard J, Carstensen B, Rossing P, Lund-Andersen H, Parving HH, et al. Years of life gained by multifactorial intervention in patients with type 2 diabetes mellitus and microalbuminuria: 21 years follow-up on the Steno-2 randomised trial. Diabetologia. 2016;59:2298–307.

Mihardja L, Soetrisno U, Soegondo S. Prevalence and clinical profile of diabetes mellitus in productive aged urban Indonesians. J Diabetes Investig. 2014;5(5):507–12.

Soebardi S, Purnamasari D, Oemardi M, Soewondo P, Waspadji S, Soegondo S. Dyslipidemia in newly diagnosed diabetes mellitus: the Jakarta primary non-communicable disease risk factors surveillance 2006. Acta Med Indones. 2009;41(4):186–90.

Pashaj A, Xia M, Moreau R. α-Lipoic acid as a triglyceride-lowering nutraceutical. Can J Physiol Pharmacol. 2015;93(12):1029–41.

Butler JA, Hagen TM, Moreau R. Lipoic acid improves hypertriglyceridemia by stimulating triacylglycerol clearance and downregulating liver triacylglycerol secretion. Arch Biochem Biophys. 2009 May;485(1):63–71.

Heinisch BB, Francesconi M, Mittermayer F, Schaller G, Gouya G, Wolzt M, et al. Alpha-lipoic acid improves vascular endothelial function in patients with type 2 diabetes: A placebo-controlled randomized trial. Eur J Clin Invest. 2010;40(2):148–54.

Ismawati, Winarto, Sari R. Pencegahan lesi aterosklerosis oleh asam alfa lipoat pada aorta mencit jantan (Mus Musculus) yang diberi diet tinggi kolesterol. J Physiol Biochem. 2012;68:307–18.

Annadurai T, Muralidharan AR, Joseph T, Hsu MJ, Thomas PA, Geraldine P. Antihyperglycemic and antioxidant effects of a flavanone, naringenin, in streptozotocin-nicotinamide-induced experimental diabetic rats. J Physiol Biochem. 2012;68(3):307–18.

Szkudelski T. Streptozotocin–nicotinamide-induced diabetes in the rat. Characteristics of the experimental model. Exp Biol Med. 2012;237(5):481–90.

Ghasemi A, Khalifi S, Jedi S. Streptozotocin-nicotinamide-induced Rat Model of Type 2 Diabetes (review). Acta Physiol ogica Hungarica. 2014;101(4):408–20.

Tangvarasittichai S. Oxidative stress, insulin resistance, dyslipidemia and type 2 diabetes mellitus. World J Diabetes. 2015;6(3):456–80.

Murugan P, Pari L. Effect of tetrahydrocurcumin on lipid peroxidation and lipids in streptozotocin-nicotinamide-induced diabetic rats. Basic Clin Pharmacol Toxicol. 2006;99(2):122–7.

Satheesh MA, Pari L. Effect of pterostilbene on lipids and lipid profiles in streptozotocin – nicotinamide induced type 2 diabetes mellitus. J Appl Biomed. 2008;(June 2014):31–7.

Nekooeian AA, Eftekhari MH, Adibi S, Rajaeifard A. Effects of pomegranate seed oil on insulin release in rats with type 2 diabetes. Iran J Med Sci. 2014;39(2):130–5.

Hammond LE, Gallagher PA, Wang SL, Hiller S, Kluckman KD, Posey-Marcos EL, et al. Mitochondrial Glycerol-3-Phosphate Acyltransferase-Deficient Mice Have Reduced Weight and Liver Triacylglycerol Content and Altered Glycerolipid Fatty Acid Composition. Mol Cell Biol. 2002;22(23):8204–14.

Carrier B, Wen S, Zigouras S, Browne RW, Li Z, Patel MS, et al. Alpha-lipoic acid reduces LDL-particle number and PCSK9 concentrations in high-fat fed obese Zucker rats. PLoS One. 2014;9(3).

Thirunavukkarasu V, Anitha Nandhini AT, Anuradha C V. Effect of α-lipoic acid on lipid profile in rats fed a high-fructose diet. Exp Diabesity Res. 2004;5(3):195–200.

Refbacks

  • There are currently no refbacks.