Analisis Gambaran Histopatologis pada Jaringan Kulit Rattus norvegicus yang Mengalami Kekerasan Tumpul Tanpa Jejas

Mohammad Tegar Indrayana, Rika Susanti, Roni Eka Syahputra, Noza Hillbertina, Dedi Afandi, Yanwirasti Yanwirasti, Nuzulia Irawati, Arni Amir

Abstract

Blunt trauma without visible skin damage is a common type of violence encountered in physical assault cases. Statistically, at RS Bhayangkara Pekanbaru, 341 cases of blunt trauma without skin damage were reported out of a total of 6,255 assault cases from 2015 to 2020. These cases present a challenge for doctors in finding physical evidence of trauma to document in medicolegal reports (Visum et Repertum). This study aims to analyze the histopathological differences, including erythrocyte extravasation, neutrophil and monocyte cell infiltration, and tissue edema, between groups subjected to blunt trauma without skin damage and a control group. Using a post-test-only group design, this study involved 30 Rattus norvegicus samples divided into three groups: control, intervention group 1, and intervention group 2. The intervention involved a 324-gram load dropped from a height of 45 cm onto the surface of the rats' skin. Histopathological examination revealed no significant differences in erythrocyte extravasation, neutrophil infiltration, or monocyte infiltration, while a significant difference was observed in tissue edema levels. 

Keywords

Blunt force injuries, erythrocyte extravasation, monocyte infiltration, neutrophil infiltration, tissue edema

References

Delfina D. Profil korban penganiayaan tanpa manifestasi jejas pada permukaan tubuh yang diperiksa di rumah sakit Bhayangkara Pekanbaru periode 2015-2020 [Skripsi]. Pekanbaru: Fakultas Kedokteran, Universitas Riau; 2021. 52 p.

Dantas MGB, Damasceno CMD, Barros VRP, et al. Creation of a contusion injury method for skeletal muscle in rats with differing impacts. Acta Cir Bras. 2017;32(5):369–73.

Desmoulin GT, Anderson GS. Method to investigate contusion mechanics in living humans. J Forensic Biomech. 2011;2:1–10.

Indrayana MT, Susanti R, Syahputtra RE, Hilbertina N, Afandi D, Yanwirasti, Irawati N, et al. Comparison of ELISA between pro-inflammatory cytokine (IL-1β, IL-6 and TNF-α) levels induced by blunt trauma without damage to the skin in Rattus novergicus. Azerbaijan Med J. 2024;64(1):1143-49.

Barington K, Jensen HE. Experimental animal models of bruises in forensic medicine - a review. Scand J Lab Anim Sci. 2015;41(14):1-8.

Kostadinova-Petrova I, Mitevska E, Janeska B. Histological characteristics of bruises with different age. Open Access Maced J Med Sci. 2017;5(7):813–7.

Parai JL, Milroy CM. Histological aging of bruising: a historical and ongoing challenge. Acad Forensic Pathol. 2015;5(2):266–72.

Langlois NEI. The science behind the quest to determine the age of bruises: a review of the English language literature. Forensic Sci Med Pathol. 2007;3(4):241–51.

Widyastuti R, et al. Pemanfaatan mencit sebagai hewan coba laboratorium. Jakarta: Erlangga; 2022.

Wuri R, Rosdianto AM, Goenawan H. Kajian Pustaka: Pemanfaatan tikus sebagai hewan model trauma tumpul (Kontusio). Indones Med Vet. 2021;10(2):338–54.

Gillette RL, Swaim SF, Sartin EA, Bradley DM, Coolman SL. Effects of a bioactive glass on healing of closed skin wounds in dogs. Am J Vet Res. 2001;62:1149–53.

Riches P. The biomechanics of bruising [thesis]. [Glasgow]: University of Glasgow; 2013. 14 p.

Akrom, Hidayati T. Trauma dan reaksi inflamasi. In: Imunofarmakologi Radang. Jakarta: Penerbit Widya Medika; 2021. p. 12-4.

Al-Saheh MA, Al-Jameel WH. Histopathological changes as tools to discriminate antemortem and post-mortem wounds in rats: prospective applications in forensic medicine. Iraqi J Vet Sci. 2022;37(1):197–204.

Khalaf AA, Hassanen EI, Zaki AR, Tohamy AF, Ibrahim MA. Histopathological, immunohistochemical, and molecular studies for determination of wound age and vitality in rats. Int Wound J. 2019;16(6):1416–25.

El-Zahed ES, Othman AMA, Ahmed SM, El-Din EAA. Role of interleukin 6 and tumor necrosis factor alpha in determination of skin wound age in human cadavers: Histopathological and immunohistochemical study. Zagazig J Forensic Med. 2020.

Sadikin M. Biokimia Darah. Edisi I. Jakarta: Penerbit Widya Medika; 2002. 127 p.

Barros MHM, Hauck F, Dreyer JH, Kempkes B, Niedobitek G. Macrophage Polarisation: an Immunohistochemical Approach for Identifying M1 and M2 Macrophages. PLoS One. 2013;8(11)

DOI: https://doi.org/10.26891/JIK.v18i2.2024.156-161

Refbacks

  • There are currently no refbacks.