Identifikasi dan Hitung Koloni Bakteri pada Tangan Perawat Kamar Operasi Instalasi Bedah Sentral (IBS) RSUD Arifin Achmad Pekanbaru Sebelum dan Sesudah Cuci Tangan dengan Antiseptik Klorheksidin Glukonat

Devi Devi, Fauzia Andrini, Kuswan Ambar Pamungkas


Handwashing is an important thing to do and the most effective way in order to prevent nosocomial infection and
infection control. The most popular surgery’s handwashing is fuerbringer method. The purpose of this study was to
know the effectivity of hand washing from the nurses that work at central operation theather Arifin Achmad General
Hospital Pekanbaru before and after washing hand with chlorhexidine gluconat antiseptic. This study method was
laboratoric descriptive with 22 samples which got from total sampling method. Bacterial was found from all sample.
Bacterial which were found from the samples before hand washing were Coagulase negative staphylococci (CONS)
(62.9%), Bacillus sp (20%), Metichillin resistant Staphylococcus aureus (MRSA) (11.4%) and Metichillin sensitive
Staphylococcus aureus (MSSA) (5.7%). Bacterial which found after doing hand wash were CONS (52.9%), Bacillus
sp (17.7%), Escherichia coli (8.8%), MRSA (11.8%), and MSSA (5.9%). The result of this bacterial colonization
area showed the reduction of bacterial colonization after doing hand wash in 90.1% sample , which mostly reduction
was happen from II degree to I (36.4%).


Surgery’s handwash, Bacterial examination, Chlorhexidine gluconat

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